Analysis Note

ControlMicrovascular endothelium

Application

Immunohistochemistry: 2 µg/mL, with paraformaldehyde fixation. Immunoblotting: 2 µg/mL

Immunoprecipitation: 1 µg/mL

FACS analysis: 2 µg/mL

ELISA: 0.5 µg/mL

Inhibition of activated human lymphocyte binding to laminin, collagen IV and fibronectin (Fabbri, 1996): 1-5 µg/mL

Optimal working dilutions must be determined by end user.

Detect Integrin α1 using this Anti-Integrin α1 Antibody, clone FB12, azide free validated for use in ELISA, FC, IH, IP, FUNC & WB.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Purified immunoglobulin in 0.02M PB, pH 7.6, 0.25M NaCl.

Format: Purified

Specificity

Reacts with the I domain (Val151 - Ala364) of human alpha1 integrin (Fabbri, 1996). Precipitates proteins of Mr 180 kDa, pI 5.9-6.0 (alpha chain) and Mr 115 kDa, pI 4.5-5.0 (beta chain) from human lymphocyte lysates (Zocchi, 1991). Does not recognize recombinant I domain of rat integrin alpha1.

Target description

130 kDa